| 紫外分光光度法在罗汉果甜甙V产品质量控制中的应用 |
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| 引用本文： | 操凤,陈敬钦,王冰清,王静晖,余波. 紫外分光光度法在罗汉果甜甙V产品质量控制中的应用[J]. 黄石理工学院学报, 2010, 26(3): 24-26. DOI: 10.3969/j.issn.1008-8245.2010.03.008 |
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| 作者姓名： | 操凤 陈敬钦 王冰清 王静晖 余波 |
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| 作者单位： | 1. 黄石理工学院,医学院,湖北,黄石,435003
2. 湖北汇特生物医药技术有限公司,湖北,黄石,435000 |
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| 摘 要： | 采用紫外分光光度法对罗汉甜牌降糖颗粒产品进行质量控制。通过对降糖颗粒中各种基质成分进行紫外扫描,确定在λ=203nm处,除罗汉果甜甙V外其他组分不对测定构成干扰,样品测定实验结果表明罗汉果甜甙V在5~200μg/mL范围内线性关系良好?R2=0.9994?,平均回收率为99.4%,RSD为0.52%(n=6)。该方法较高效液相色谱法(HPLC)经济节约、简便易操作、快速准确,可作为罗汉果甜甙V产品质量控制的手段。
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| 关 键 词： | 罗汉果甜甙V 质量控制 紫外分光光度法 |
Application of UV in the Quality Control of Duicoside V-based Product |
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| CAO Feng,CHEN Jingqin,WANG Bingqing,WANG Jinghui,YU Bo. Application of UV in the Quality Control of Duicoside V-based Product[J]. Journal of Huangshi Institute of Technology, 2010, 26(3): 24-26. DOI: 10.3969/j.issn.1008-8245.2010.03.008 |
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| Authors: | CAO Feng CHEN Jingqin WANG Bingqing WANG Jinghui YU Bo |
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| Affiliation: | 1 School of Medicine,Huangshi Institute of Technology,Huangshi Hubei 435003,2 Hubei Fyto Biopharmaceutical Co.Inc.,Huangshi Hubei 435003) |
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| Abstract: | UV was applied to the quality control of Duicoside V-based product.Other components except Duicoside V demonstrated no absorption at λ203 by UV scanning.Results showed that the linear scope was on the range of 5～200μg/ml（R2=0.9994）,Average recovery was 99.4%,RSD was 0.52%（n=6）.The proposed UV method was ecnomical,simple,time-saving and pricise,which can be used in the quality control of Duicoside V-based product. |
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| Keywords: | duicoside V quality control UV |
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