氘代同位素内标-气相色谱-质谱法测定胆固醇含量鉴别地沟油的研究

采用氘代同位素稀释气相色谱-质谱方法,分析了不同种类植物油和不同来源地沟油中胆固醇含量。本方法的灵敏度和稳定性明显高于文献报道的方法,可以明显鉴别出不同种类的植物油以及植物油与地沟油胆固醇含量的差别,根据测定结果偏离情况可以进行是否添加地沟油的判定。利用该判别依据,参加了两次卫生部组织的地沟油盲样考核,阳性准确率均在90%以上,表明该方法可以作为掺杂地沟油鉴别方法之一。

Determination of Cholesterol in Hogwash Oils by Deuterated Internal Stand Method Coupled with Gas Chromatography-Mass Spectrometry

Using deuterated internal stand,a gas chromatography-mass spectrometric method（GC-MS） was developed for the identification and quantitative determination of cholesterol in various vegetable oils and hogwash oils.The sensitivity and stability of the method was significantly higher than reported in the literature.The differences of cholesterol content between vegetable oils and hogwash oils can be clearly identified and whether the oil is hogwash oil or not can be made according to the deviations.The method was applied to distinguish hogwash oils from good vegetable oils in 2 blind assessment tests.The both tests were of more than 90% accuracy and it is showed that this method can be applied to differentiate hogwash oils from good vegetable oils.