| 人肝癌BEL7404／ADM耐药株的建立及生物学特性的评价 |
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| 引用本文： | 曹芳,周素芳.人肝癌BEL7404／ADM耐药株的建立及生物学特性的评价[J].黄石理工学院学报,2011(4):56-59. |
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| 作者姓名： | 曹芳 周素芳 |
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| 作者单位： | 黄石理工学院化学与材料工程学院;广西医科大学生物化学与分子生物学教研室; |
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| 基金项目： | 广西研究生创新教育计划资助(项目编号:2006105981001N01) |
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| 摘 要： | 建立人肝癌BEL7404／ADM耐药株，并检测该耐药株的生物学特性。通过盐酸阿霉素（adria-mycin，ADM）长期筛选人肝癌细胞株BEL7404，建立阿霉素耐药株BEL7404／ADM。应用M1T法对该细胞株药物敏感性和倍增时间进行检测，用流式细胞仪对其细胞周期和P-糖蛋白（P—glycoprotein，P—gp）功能进行研究。用浓度梯度递增法对BEL741M细胞连续作用10个月，得到人肝癌BEL7404／ADM耐药株。经检测该耐药株IC50是BEL7404细胞株的68．7倍，倍增时间延长了6．49h，两者细胞周期无明显差异性，P—gP活性高于BEL7404细胞株。成功建立了人肝癌BEL7404／ADM耐药株，该耐药株的生物学特性明显不同于BEL7404细胞株；耐药性与P—gp的高表达有关。
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| 关 键 词： | 肝癌细胞 耐药性 盐酸阿霉素 |
Establishment of Human Hepatocellular Carcinoma BEL7404/ADM Drug-Resistant Line and Biological Characteristics Evaluation |
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| CAO Fang ZHOU Sufang.Establishment of Human Hepatocellular Carcinoma BEL7404/ADM Drug-Resistant Line and Biological Characteristics Evaluation[J].Journal of Huangshi Institute of Technology,2011(4):56-59. |
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| Authors: | CAO Fang ZHOU Sufang |
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| Institution: | CAO Fang1 ZHOU Sufang2(1 School of Chemical and Materials Engineering,Huangshi Institute of Technology,Huangshi Hubei 435003,2 Teaching and Research Office of Biochemistry and Molecular Biology,Guangxi Medical University,Nanning Guangxi 530021) |
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| Abstract: | Objective: to establish human hepatocellular carcinoma adriamycin(ADM)-resistant strain BEL7404/ADM and to test its biological characteristics.Methods: The BEL7404 line was induced to form an adriamycin-resistant cell line(BEL7404/ADM) by exposing it to increase gradually concentration of ADM.MTT assay was used to measure drug sensitivity and cell growth.Flow cytometric assay was employed to determine cell cycle and drug influx-and-efflux.Results:The resistant index of BEL7404/ADM cell lines was as 68.7 tim... |
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| Keywords: | hepatocellular carcinoma resistant Adriamycin |
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