复方血栓通系列品种中四种皂苷通用检测方法研究

目的统一复方血栓通颗粒、片、滴丸和软胶囊中三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量测定方法。方法采用高效液相色谱法,使用Thermo ODS2(5μm,150.0 mm×4.6 mm)色谱柱,柱温为20℃,以乙腈-水为流动相进行梯度洗脱,流速为1.0 ml/min,检测波长为203 nm。结果三七皂苷R1在0.0518~2.5900(r=0.9996)、人参皂苷Rg1在0.2090~8.3588(r=0.9991)、人参皂苷Re在0.0507~2.5371(r=0.9990)、人参皂苷Rb1在0.2044~8.1752(r=0.9995)范围内与峰面积线性关系良好;精密度、重复性、稳定性及加样回收率结果均能满足分析要求。结论该方法简单可行,为复方血栓通品种中三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1含量测定方法的统一提供了可靠的依据。

Universal Method for the Determination of four saponins in Fufang XueShuanTong Preparations

Objective To unified the method for the content determination of Notoginsenoside R1,Ginsenoside Rg1,Ginsenoside Re,Ginsenoside Rb1 of Fufang Xueshuantong granules,tablets,dripping pils and soft capsules. Methods By HPLC method;Notoginsenoside R1,Ginsenoside Rg1,Ginsenoside Re,Ginsenoside Rb1were analyzed on an Thermo ODS2（5 μm,150.0 mm×4.6 mm）column at 20℃;The gradient elution was acetonitrile-water at the flow rate of 1.0ml/min;The detection wavelength was 203 nm.Results Notoginsenoside R1,Ginsenoside Rg1,Ginsenoside Re,Ginsenoside Rb1 al have good linearity in the range of 0.0518-2.5900（r=0.9996）,0.2090-8.3588（r=0.9991）, 0.0507-2.5371（r=0.9990）,0.2044-8.1752（r=0.9995）with satisfied precision,repetition,stability and recovery.Conclusion This method is simple,feasible,and can offer the reliable basis for unification of the standards.