高效液相色谱法测定辣椒油中苏丹红的含量

本文对国家标准《食品中苏丹红染料的检测方法高效液相色谱法》(GB/T 19681-2005)中利用高效液相色谱法测定辣椒油中苏丹红含量的方法进行优化,使方法更加简便快捷,且满足实验要求。以正己烷为提取溶剂,提取液经ProElut SDH固相萃取柱净化,经高效液相色谱柱分离,外标法定量。优化后的方法中,苏丹红Ⅰ~Ⅳ在0.16~2.56μg/mL的浓度范围内线性关系良好,苏丹红Ⅰ~Ⅳ的检出限分别为0.010 7μg/kg、0.011 1μg/kg、0.011 9μg/kg、0.011 0μg/kg,加标回收率为89.21%~95.69%。优化后的实验方法易于操作,能够缩短实验时间,有良好的回收率,更适合检测辣椒油中苏丹红的含量。

Determination of Sudan Red in Chili Oil by HPLC

The national standard GB/T 19681-2005 was optimized by high performance liquid chromatography to determine the content of Sudan red in chili oil,making the method simple and fast,and meeting the experimental requirements.Using n-hexane as the extraction solvent,the extract was purified by ProElut SDH solid phase extraction column,separated by high performance liquid chromatography column,and quantified by external standard method.In the optimized method,Sudan Red I Sudan Red IV has a good linear relationship in the concentration range of 0.162 56μg/kg,and the detection limits of Sudan Red I Sudan Red IV are 0.010 7μg/kg,0.011 1μg/kg,0.011 9μg/kg,0.011 0μg/kg,the recovery rate of spiked is 89.21%,95.69%.The optimized experimental method is easy to operate,can shorten the experimental time,has a good recovery rate,and is more suitable for the detection of Sudan red content in chili oil.