高效液相色谱法测定香烤瓜子中糖精钠含量的不确定度评估

目的：为提高检验结果的准确性,确定检验过程中的关键影响因素,对高效液相色谱法测定炒货中糖精钠含量进行不确定度评估。方法：依据国家标准GB 5009.28—2016采用高效液相色谱法对香烤瓜子中糖精钠的含量进行测定,分析影响不确定度的因素,根据CNAS—GL006:2019对糖精钠不确定度评估建立数学模型,以JJF 1059.1—2012为依据对检验过程中的不确定因素进行评估,并计算合成相对标准不确定度和扩展不确定度。结果：高效液相色谱法测定香烤瓜子中糖精钠含量的不确定度结果表示为(1.583±0.188)g·kg^-1,k=2(p=95%)。结论：香烤瓜子中糖精钠含量测定的不确定度主要来源于标准溶液的配制和标准工作曲线拟合。在实验中应选择精度较高的量器,减少随机误差,降低不确定因素对检验结果准确性的影响。

The Evaluation of Uncertainty of Sodium Saccharine Content in Roasted Sunflower Seeds by High Performance Liquid Chromatography

Objective:In order to improve the accuracy of the test results,determine the key influencing factors during the experiment,and to evaluate the uncertainty of sodium saccharin content in roasted sunflower seeds by high performance liquid chromatography.Method:According to the national standard GB 5009.28—2016,the content of sodium saccharin in roasted sunflower seeds was determined by high performanceliquid chromatography and the source of uncertainty was analyzed.According to CNAS—GL006:2019,a mathematical model was established for the uncertainty evaluation of saccharin sodium,and the uncertainty factors in the inspection process were evaluated based on JJF 1059.1—2012,and the synthetic relative standard uncertainty and expanded uncertainty were calculated.Result:The uncertainty of sodium saccharin content in roasted sunflower seeds by high performanceliquid chromatography was expressed as(1.583±0.188)g·kg^-1,k=2(p=95%).Conclusion:The uncertainty of sodium saccharin content in roasted sunflower seeds mainly affected from the preparation of standard solutions and the fitting of standard curves.In the experiment,a measuring instrument with higher precision should be selected to reduce random errors and reduce the influence of uncertainty on the accuracy of test results.