枯草芽孢杆菌产腺苷补料发酵工艺的研究

目的：本文采用枯草芽孢杆菌(Bacillus subtilis)为发酵菌种,考察发酵液中次黄嘌呤前体物质的质量浓度与发酵产率的关系、次黄嘌呤前体物质加入方式与腺苷产量的关系,以研究提高腺苷发酵水平的补料发酵工艺。方法：利用摇瓶、50 L发酵罐、10 m发酵中试罐,整合补料与溶解氧的发酵过程调控,进行逐级放大试验。结果：在10 m³中试发酵罐进行补料工艺放大,流加补糖含9 g·L^-1的次黄嘌呤,并通过阶段控制溶氧条件,腺苷产量达到35.06 g·L^-1。结论：次黄嘌呤的添加对腺苷的生物合成有促进作用,分阶段溶氧控制连续流加次黄嘌呤工艺腺苷产量有明显的优势,腺苷补料发酵工艺的研究为工业化应用提供参考。

Study on Fed-Batch Fermentation of Adenosine Production by Bacillus subtilis

Objective:Bacillus subtilis was used as the fermentation strain to investigate the relationship between the mass concentration of hypoxanthine precursors in the fermentation broth on the fermentation yield,and the relationship between the addition method of hypoxanthine precursors on the yield of adenosine,so as to study the fed-batch fermentation process to improve the fermentation level of adenosine.Method:The shake flasks,50 L fermentation tanks,and 10 m³ fermentation pilot tanks were used to integrate the fermentation process regulation of feeding and dissolved oxygen,and conduct a step-by-step scale-up experiment.Result:The feeding process was enlarged in a 10 m³ pilot-scale fermenter,the feed-supplemented sugar contained 9 g·L^-1hypoxanthine,and the dissolved oxygen conditions were controlled by stages,and the adenosine yield was 35.06 g·L^-1.Conclusion:The addition of hypoxanthine can promote the biosynthesis of adenosine,and the step-by-step dissolved oxygen control process of continuous flow addition of hypoxanthine has obvious advantages in adenosine yield.The research on adenosine fed-batch fermentation process provides a reference for industrial application.