优化高效液相色谱-串联质谱法检测水产品中酰胺醇类药物残留 |
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作者姓名: | 林敏霞 徐媛原 钟仕花 |
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作者单位: | 广东省深圳市质量安全检验检测研究院,广东 深圳 518000 |
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摘 要: | 目的:对GB/T 20756—2006水产品中酰胺醇类残留量的测定方法进行了优化.方法:经匀质的水产品样品,加10 mL乙腈涡旋振荡,一次提取和除杂后,取4 mL提取液,35℃水浴氮吹近干,用20%乙腈水1 mL定容,过0.22μm滤膜.样液用高效液相色谱进行分离,电喷雾串联四级杆质谱进行检测,以氯霉素-D5为内标物进...
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关 键 词: | 高效液相色谱串联质谱 酰胺醇类 水产品 |
Optimization of High Performance Liquid Chromatography-Tandem Mass Spectrometry for the Detection of Amide Alcohol Drug Residues in Aquatic Products |
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Authors: | LIN Minxia XU Yuanyuan ZHONG Shihua |
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Institution: | (Shenzhen Institute of Quality and Safety Inspection and Testing,Shenzhen 518000,China) |
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Abstract: | Objective:to optimize the method for the determination of amidol residues in aquatic products(GB/T 20756—2006).Methods:The homogenized aquatic product sample was added with 10 mL acetonitrile for vortex oscillation,followed by primary extraction and impurity removal.Then 4 mL of the extractive solution was taken,blown nearly dry under nitrogen in a 35℃water bath,fixed in volume with 1 mL of 20%acetonitrile water,and passed through a 0.22μm filter membrane.The sample was separated by high performance liquid chromatography(HPLC)and detected by electrospray ionization tandem mass spectrometry with chloramphenicol-D5 as the internal standard for quantification.Results:The linear range of the method was from 0.10μg·kg-1 to 6.00μg·kg-1,the correlation coefficients are all greater than 0.999.The recovery of the method was in the range of 83.2%~93.6%,and the relative standard deviation was in the range of 2.7%~9.9%.Conclusion:This method has the advantages of high sensitivity and good accuracy.Compared with GB/T 20756—2006,the pre-treatment steps were simplified and the recovery rate was stable.Through verification,the method is suitable for the detection of amide alcohol residues in aquatic products. |
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Keywords: | high performance liquid chromatography tandem mass spectrometry amide alcohols aquatic products |
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