茶树菇多糖提取工艺优化及抗氧化活性研究 |
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作者姓名: | 高雅倩 马诗经 林丽 杨宜婷 |
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作者单位: | 无限极(中国)有限公司,广东 广州 510623;广州市康伦生物技术有限公司,广东 广州 510000 |
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摘 要: | 以茶树菇为原料,通过超微粉碎辅助热水浸提工艺提取茶树菇多糖.以多糖得率为评价指标,在单因素试验的基础上,通过正交试验对茶树菇多糖提取工艺进行优化;进一步测定不同浓度乙醇醇沉得到的茶树菇多糖C1、C2对DPPH自由基清除率的影响.结果表明,茶树菇多糖最佳提取工艺为液料比为30:1(mL:g),提取温度100℃,提取时间3...
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关 键 词: | 茶树菇多糖 超微粉碎 醇沉 抗氧化 |
Study on the Optimization of Extraction Technology of Agrocybe aegerita Polysaccharide and Its Antioxidation Activity |
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Authors: | GAO Yaqian MA Shijing LIN Li YANG Yiting |
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Institution: | (Infinitus(China)Co.,Ltd.,Guangzhou 510623,China;Guangzhou Conney Biotechnology Co.,Ltd.,Guangzhou 510000,China) |
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Abstract: | Agrocybe aegerita polysaccharide was extracted from Agrocybe aegerita by ultra-fine grinding assisted hot water extraction process.Taking the polysaccharide yield as the evaluation index,the extraction process ofAgrocybe aegerita polysaccharide was optimized by orthogonal test on the basis of single factor test;The effects of Agrocybe aegerita polysaccharide C1 and C2 obtained by ethanol precipitation of different concentrations on DPPH radical scavenging were further determined.The results showed that the optimal extraction conditions were liquid/material ratio of 30:1(mL:g),extraction temperature of 100℃,extraction time of 3 h,extraction once,under these conditions,the yield of Agrocybe aegerita polysaccharide could reach 11.96%±1.06%.In addition,Agrocybe aegerita polysaccharides C1 and C2 had good scavenging effect on DPPH free radicals,presenting a good dose-effect relationship. |
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Keywords: | Agrocybe aegerita polysaccharide superfine pulverizing technique ethanol precipitation antioxidation |
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