高效液相色谱法测量牛奶中山梨酸的过程优化研究

目的:研究沉淀剂、检测波长、操作流程对牛奶回收率的影响,查找过程检测优化条件.方法:通过对《食品安全国家标准食品中苯甲酸、山梨酸和糖精钠的测定》(GB 5009.28—2016)步骤进行梳理研究,通过单因子实验确定过程优化参数.结果:使用2％乙酸铅较亚铁氰化钾和乙酸锌组合回收率高,230 nm和245 nm波长下牛奶中...

Optimization of Sorbic Acid Determination in Milk by High Performance Liquid Chromatography

Objective: To study the effects of precipitant, detection wavelength and operation process on milk recovery, and to find the optimal conditions for process detection. Methods: The pretreatment and instr μmental analysis steps of GB 5009.28—2016 were combed and studied, and the process optimization parameters were determined by single factor experiment. Results: The recovery rate of 2% lead acetate was higher than that of potassi μm ferrocyanide and zinc acetate. There was no significant difference in the recovery rate of sorbic acid in milk at 230 nm and 245 nm. The operation steps were optimized to make the pretreatment more simple and easy to operate. The recovery rate was slightly improved, but the increase was not large. Conclusion: Using 2% lead acetate as precipitant and optimizing the analysis steps, the detection accuracy of sorbic acid in milk can be improved to 98%, and the pretreatment operation is simple.