| 猪肉中克伦特罗、沙丁胺醇、土霉素、四环素残留量的HPLC同时测定方法研究 |
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| 引用本文： | 金雁,姚家彪,郭隽,蒋施,赵颖,潘伟,钟钰.猪肉中克伦特罗、沙丁胺醇、土霉素、四环素残留量的HPLC同时测定方法研究[J].检验检疫科学,2008,18(5):30-33. |
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| 作者姓名： | 金雁 姚家彪 郭隽 蒋施 赵颖 潘伟 钟钰 |
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| 作者单位： | 沈阳出入境检验检疫局,辽宁沈阳,110016 |
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| 摘 要： | 目的]建立高效液相色谱法(HPLC)同时测定猪肉样品中克伦特罗、沙丁胺醇、土霉素、四环素残留量的检测方法.方法]样品用0.01 mol/L EDTA-2Na、0.3%磷酸溶液、高氯酸(1:1)混合溶液提取,上清液过C18固相萃取柱净化,采用高效液相色谱分离,二极管阵列检测器(DAD)检测,外标法定量.结果]克伦特罗、沙丁胺醇在0.04～10μg/mL范围内线性关系良好.相关系数为0.99992～0.99993.在0.001～0.05mg/kg添加浓度范围内,平均回收率在70.3%～94.3%之间,相对标准偏差为1.52%～5.85%,土零素、四环素在0.5～10μg/mL范围内线性关系良好,相关系数为0.99993～0.99997.在0.02～0.5mg/kg添加浓度范围内,平均回收率在64.1%～94.2%之间,相对标准偏差为1.23%～4.53%.结论]本法简便、快速、定量准确、精密度商.适用于猪肉样品中克伦特罗,沙丁胺醇、土霉素、四环素残留量的同时检测.
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| 关 键 词： | 克伦特罗 沙丁胺醇 土霉素 四环素 残留检测 |
SIMULTANEOUS DETERMINATION OF CLENBUTEROL,SALBUTAMOL,OXYTETRACYCLINE AND TETRACYCLINE RESIDUES IN PORK BY HPLC |
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| JIN Yan,YAO Jiabiao,GUO Jun,JIANG Shi,ZILAO Ying,PAN Wei,ZHONG Yu.SIMULTANEOUS DETERMINATION OF CLENBUTEROL,SALBUTAMOL,OXYTETRACYCLINE AND TETRACYCLINE RESIDUES IN PORK BY HPLC[J].Inspection and Quarantine Science,2008,18(5):30-33. |
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| Authors: | JIN Yan YAO Jiabiao GUO Jun JIANG Shi ZILAO Ying PAN Wei ZHONG Yu |
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| Institution: | JIN Yan,YAO Jiabiao,GUO Jun,JIANG Shi,ZHAO Ying,PAN Wei,ZHONG Yu (Shenyang Entry-Exit Inspection , Quarantine Bureau,Shenyang,Liaoning,110016,China) |
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| Abstract: | High performance liquid chromatographic method(HPLC)has been developed for the simultaneous determination of clenbuterol,salbutamol,oxytetracycline and tetracycline in pork.The samples were extracted with 0.01 mol/L disodium ethylenediamine tetraacetate,0.3%phosphate,and perchloric acid(1:1).Sample clean-up was performed on a C18 solid phase extraction column.The analytes were separated by HPLC with diode array detector and quantified using external standard calibration curves.The linear ranges of clenbuter... |
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| Keywords: | HPLC |
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