| 鸡冠花乙醇提取物的 HPLC 指纹图谱研究 |
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| 引用本文： | 张海晶,金兰,于金平,陈大忠.鸡冠花乙醇提取物的 HPLC 指纹图谱研究[J].黑龙江商学院学报,2014(1):18-20,24. |
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| 作者姓名： | 张海晶 金兰 于金平 陈大忠 |
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| 作者单位： | [1]黑龙江中医药大学中医药研究院,哈尔滨150040 [2]东北农业大学食品学院,哈尔滨,150030 |
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| 摘 要： | 建立鸡冠花的HPLC指纹图谱分析方法，为科学评价及有效控制其质量提供可靠的依据和手段．采用HPLC法测定10批鸡冠花样品的指纹图谱，色谱柱为Dikma C18（250 mm×4．6 mm，5μm），流动相为乙腈-0．1％磷酸水溶液，梯度洗脱，流速为1．0 mL/min，检测波长为260 nm，分析时间90 min．建立共有模式，以相关度评价图谱的相似性．10批次鸡冠花样品色谱指纹图谱共有16个共有峰，相似度在0．9以上，不同批次鸡冠花质量相似性较好．该方法可用于鸡冠花质量评价．
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| 关 键 词： | 鸡冠花 指纹图谱 高效液相色谱法 Celosia cristata L |
Study on HPLC fingerprint of Celosia cristata L.ethanol extract |
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| ZHANG Hai-jing,JIN Lan,YU Jin-ping,CHEN Da-zhong.Study on HPLC fingerprint of Celosia cristata L.ethanol extract[J].Journal of Harbin Commercial University(Natural Sciences Edition),2014(1):18-20,24. |
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| Authors: | ZHANG Hai-jing JIN Lan YU Jin-ping CHEN Da-zhong |
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| Institution: | 1. Research Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China; 2. School of Food Science, Northeast Agricultural University, Harbin 150030, China) |
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| Abstract: | HPLC fingerprint of Celosia cristata L .was established to provide a reliable meth-od for the science appraisal and active control its quality .The chromatographic conditions were as follows：Dikma C18 chromatobar column （250 mm ×6 mm, 5 μm）, gradient elu-tion with acetonitrile -0.1% phosphoric acid solution , volume flow rate of 1.0 mL/min, under the detection wave length of 257 nm, and record time of 90 min.Data was analyzed to compare the similarity of the samples for identifying the main chromatographic peaks further-more .The chromatographic fingerprint of 10 batches of Celosia cristata L .showed 16 charac-teristic peaks, with the similarity above 0.9.The qualities of Celosia cristata L .in different batches were in a good similarity .The method can be applied to quality of Celosia cristata L . |
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| Keywords: | fingerprint spectrum HPLC |
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